Day 1 :
Keynote Forum
Omar M Amin
Parasitology Center Inc., USA
Keynote: Understanding parasites and cancer in the United States
Time : 10.00-10:45
Biography:
Abstract:
This Power Point presentation is based on our work at Parasitology Center, Inc. (PCI), in Scottsdale, Arizona, USA and covers the diagnosis, pathology, relationships with cancer and treatment of human parasitic infections in the United States based on our own patient history and testing. The conceptual thesis and practical observations of extensive damage of parasites to human tissues and the initiation of host defense strategies causing out of control cell divisions leading to metastasis is emphasized. A brief introduction to laboratory procedures, misdiagnoses/mistreatment and impact on public health, especially cancer is made. A systematic treatment of protozoan and helminth (worm) parasites follows, emphasizing epidemiology and exposure, symptoms and gross pathology. Herbaland allopathic remedies including our own anti-parasitic herbal product freedom/ cleanse/restore are presented. All topics are illustrated with labeled pictures of the various kinds of parasites and their gross pathology in human tissues, when applicable. The presentation is followed by a brief discussion of case histories and treatment of intestinal pathogenic bacteria that usually cause GI symptoms similar to those caused by intestinal parasites.
Keynote Forum
Hridaya Shanker Singh
C C Singh University, India
Keynote: Monogenean taxonomy: A bird’s eye view
Time : 11:05-11:50
Biography:
Hridaya Shanker Singh is present Pro- Vice-Chancellor, Ch. Charan Singh University, Meerut; Chairman, IQAC, Ch. Charan Singh University, Meerut and Head, Department of Zoology, Ch. Charan Singh University, Meerut. He is having life Memberships and awards in Helminthological Society of India, Indian Society of Parasitology, Society of Parasitology and Applied Animal Biology, Indian Association for the Advancement of Veterinary Parasitology, Zoological Society of India, Indian Science Congress Association.
Abstract:
Studies about taxonomy and biodiversity of helminthes (including Monogeneans) in India were started from the middle of 20th century by helminthologists who came to this country on medical or military deputation from foreign lands. As regard biodiversity studies related to Indian Monogeneans is concerned, it also started w.e.f., 1940s by workers like - Chauhan, Thapar, Jain, Unnithan, Gupta (SP), Gupta (NK), Agarwal (GP), Ramalingam, Tripathi, Gussev etc. Recently, Pandey and Agrawal compiled a comprehensive account of known species of Monogeneans from India which is estimated to be about 300, which is far from complete. The present talk deals with the taxonomic history of monogeneans in India. It includes the taxonomic tools adopted by Indian helminthologists in the study of monogenean systematics viz., morphological taxonomy, cytotaxonomy, biochemical taxonomy and molecular taxonomy. Indian subcontinent is blessed with five major river systems of India viz., Ganga, Brahmaputra, Indus, East coast and West coast river systems. These rivers are long and are further fed strengthened by many large and important tributaries. Moreover, many small seasonal and perennial rivers also use to contribute to these river systems separately. The investigator is actively engaged in the study of freshwater monogeneans since 1980. The present study reveals that about 35.45% fishes have so far been investigated for monogenean infection in general and still 74% are remaining that are not screened. Helminth parasites, particularly monogeneans lead greater losses on account of having direct life cycle, which can be completed easily in a closed system of fish culture. If we stick to one host one parasite rule, there exists a very big gap as far as state of our knowledge related to biodiversity of freshwater monogeneans are concerned. It is clear from the present review that much remains to be done about this group within India in general and throughout the globe in particular with newer tools including molecular studies.
- Parasitology | Zoonotic Infectious Diseases | Airborne, Vector borne & Contact Infectious Diseases | Paediatric Infectious Diseases | Nosocomial Parasitic Infections
Location: Akdeniz 1
Chair
Ramadan A M Ramadan
Agriculture Research Center, Egypt
Session Introduction
Meysam Sharifdini
Guilan University of Medical Sciences, Iran
Title: Helminthes in Feral raccoon (Procyon lotor) as an alien species in Iran
Time : 11:50-12:20
Biography:
Meysam Galyan Sharif Dini has completed his PhD in Medical Parasitology from Tehran University of Medical Sciences, Iran. He is currently working as an Assistant Professor of the Department of Medical Parasitology and Mycology at School of Medicine, Guilan University of Medical Sciences, Iran. His major research interest includes Strongyloidiasis and Trichostrongyliasis. He has published more than 25 papers in reputed journals and also has served as Reviewer of several national and international journals.
Abstract:
Raccoon (Procyon lotor) is a native mammal in North and Central America. At present, as a result of escapes and deliberate introductions in the mid-20th century, the raccoon is now distributed in several European and Asian countries such as Japan, Germany and Iran. Objective of this study was to determine prevalence of gastrointestinal and tissue helminths from feral raccoons in Guilan province. During 2015-2017, a total of 30 feral raccoons including 12 males and 18 females were collected form Guilan province, northern Iran. The gastrointestinal tracts and tissues such as lung, liver and muscles were examined for presence of helminths. 20 raccoons (66.7%) were found infected with five intestinal helminth species. The prevalence of infection with Strongyloides procyonis Little, 1966 (Nematoda) was 63.3%, Plagiorchis koreanus Ogata, 1938 (Trematoda) (13.3%), Centrorhynchus sp. Luhe, 1911 (Acanthocephala) (10.0%), Camerostrongylus didelphis Wolfgang, 1951 (Nematoda) (3.3%), and Spirocerca lupi Rudolphi, 1809 (Nematoda) (3.3%). No larvae or adult worms were found in other tissues of the examined raccoons. No larvae or adult worms were found in the tissues of examined raccoons. In current study, most of the raccoons were infected with S. procyonis. Concerning public health importance of zoonotic parasites transmittable through raccoons, the rapid control and decrease of population and distribution of raccoons in Iran is suggested.
Ozer Akgul
Istanbul Aydın University, Turkey
Title: High association of intestinal parasites with cancer and organ transplant recipient patients in Turkey
Time : 12:20-12:50
Biography:
Abstract:
Abdalla Fadlalla Azrug Ahmed
Agriculture and Marine Resources Affairs, Bahrain
Title: Prevalence and economic impact of haemonchosis among sheep in some semi-closed system farms in Bahrain
Time : 13:40-14:10
Biography:
Abstract:
Layla Omran Elmajdoub
Misurata University, Libya
Title: Genetic variants within the strains of Echinococcus granulosus by mitochondrial DNA sequencing
Time : 14:10-14:40
Biography:
Abstract:
Ramadan A M Ramadan
Agriculture Research Center, Egypt
Title: Assessment of KAP regarding HIV testing among military personnel in Omdurman military area 2017 - Sudan
Time : 14:40-15:10
Biography:
Ramadan A M Ramadan is currently working in the Fish Diseases Department at Central Lab. For Aquaculture Research (El-Abbassa), Agriculture Research Center, Egypt. His Present research or professional specialties are in Parasitogy of freshwater and marine fish, crustaceans and molluscs.
Abstract:
- Video Presentation
Location: Akdeniz 1
Session Introduction
Adam Suliman
Communicable Diseases Control at Military Medical Services, Sudan
Title: Assessment of KAP regarding HIV testing among military personnel in Omdurman military area 2017-Sudan
Time : 15:30-16:00
Biography:
Adam Suliman has completed his BSc from the University of Khartoum, Faculty of Public and Environmental Health, MSc in Public Health from the same University. He is the Director of Communicable Diseases Control Department, at Military Medical Services G. Admin. He has published 2 papers in reputed journals.
Abstract:
- Parasitic Diseases & Plant Pathogens | Infectious Diseases and Health Care | Laboratory Diagnosis - Therapeutic Parasitology
Location: Akdeniz 1
Chair
Fatma Hamed Shalan
Menofia University, Egypt
Session Introduction
Omar M Amin
Parasitology Center Inc., USA
Title: Variability of Acanthocephalans
Time : 10:00-11:00
Biography:
Abstract:
Biography:
Abstract:
Biography:
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Biography:
Abstract:
Asmaa Ibrahim Nasr
Cairo University, Egypt
Title: Helicobacter pylori and enteric parasites co-infection among Egyptian children: Estimated risks, and predictive factors
Time : 11:20-11:50
Biography:
Motivated student currently working towards degree in molecular biology (molecular genetics and cytogenetics). Adept at prepping resources, equipment, and materials for research. Extensive background in investigating molecular parasitology and microniology. Seeking to secure rewarding Research Assistant role to facilitate Research for thesis. Efficient Research Assistant able to complete a wide range of support tasks under strict schedules. Systematic and meticulous in all work. Eager to contribute to infectious diseases research. Skilled Research Assistant knowledgeable about conventional PCR, Real time PCR and Elisa.
Abstract:
H.pylori and intestinal parasites are known for their high prevalence in children. Both of them infect the gastrointestinal tract with overlapping clinical pictures. This study was conducted to determine H.pylori prevalence and its association with intestinal parasites in children, moreover to estimate risk and predictive factors for their detection in stool samples. Single fecal samples were collected from 226 Egyptian pediatric patients (125 diarrheic and 101 non-diarrheic) attending gastroenterology outpatients’ clinics, from February 2016 to June 2017. All stool specimens were microscopically examined to search for ova and parasites. Copro-DNAs detection of H.pylori and Cryptosporidium were performed using nested-PCR assays.H. pylori was detected molecularly in 36.8% of the total study population, with a higher prevalence in diarrheic than in non-diarrheic children. Intestinal parasites were detected in 27.4% of the total study population, of these, 43.9% had co-existence with H.pylori colonized patients and was significantly associated with Cryptosporidium spp. and G.intestinalis. Estimated risk of the presence of H.pylori in January. Our data provide a better understanding of the epidemiology of H.pylori infection when associated with intestinal parasites. H.pylori co-existence with G.intestinals and Cryptosporidium may suggest the association of H.pylori infection with markers of fecal exposure. Whether H.pylori provides favorable conditions for intestinal parasitosis or vice versa, still further investigations are needed with an emphasis upon determining correlation with gut microbiomes.
Zahra Hesari
Guilan University of Medical Sciences, Iran
Title: In vitro effects of Pumpkin (Cucurbita moschata) seed extracts on Echinococcus granulosus protoscoleces
Time : 11:50-12:20
Biography:
Abstract:
Rifat Ullah Khan
The University of Agriculture, Pakistan
Title: Anticoccidial effect of garlic (Allium sativum) and ginger (Zingiber officinale) against experimentally induced coccidiosis in broiler chickens
Time : 12:20-12:50
Biography:
Abstract:
Hridaya Shanker Singh
C. C. Singh University, India
Title: A review of Monogenean diversity in India: Pathogens of fish diseases
Time : 13:40-14:10
Biography:
Abstract:
Fatma Hamed Shalan
Menofia University, Egypt
Title: Mesenchymal stem cell as a cure for brain damage induced by chronic Toxocara canis infection in an experimental mouse model
Time : 14:10-14:40
Biography:
Abstract:
Neurotoxocariasis is a serious condition linked to the reduced cognitive function in children and some neurodegenerative diseases. Unfortunately, the available drugs for its treatment are with variable results. Mesenchymal stem cells (MSCs) have been used in experimental and clinical trials and it gave promising therapeutic results. Therefore, this study was designed using forty T. canis-infected albino mice (1000 eggs/mouse, orally) and a control group (GI) of ten healthy mice. The infected groups were GII: infected non-treated (control group), GIII: albendazole-treated (100 mg/kg/d once orally for 5 successive days), GIV: MSCs-treated (3 x 106 MSCs in 0.1 mL of PBS via the tail vein) and GV: albendazole + MSCs-treated. Treatment was commenced 6 weeks p.i. and the experiment was terminated four weeks after treatment. The brain tissue of each mouse was subjected for histopathological, immunohistochemical studies (caspase-3, TGF-β), detection or T. canis DNA by real-time PCR and gene expression the biomarkers of brain damage (S100B, GFAP) by RT-PCR. Moreover, homing of iron oxide-labelled MSCs in brain tissues was assessed by Prussian blue stain. The brain tissues of GII showed numerous T. canis larvae, significant congestion, thickening of arterioles, inflammatory infiltrate and gliosis associated with marked immunohistochemical expression of TGF-β and caspase-3 as well as marked S100B and GFAP gene expression. Significant improvements of the previous parameters and T. canis DNA were recorded in all the treated groups. However, the best results were obtained with combined albendazole + MSCs therapy. Thus, MSCs could be considered in the treatment of chronic neurotoxocariasis.
- Poster Presentations
Location: Akdeniz 1
Session Introduction
Cornelia Voigtlander
Institute of Medical Biotechnology, Germany
Title: The role of MORN1 in the intraerythrocytic life cycle of Plasmodium falciparum
Time : INF-01
Biography:
Abstract:
Due to increasing drug resistance of Plasmodium falciparum, a search for novel drug targets is of great importance. In this respect, the nuclear division cycles of the malaria parasite are of particular interest, since they differ from traditional mitosis in several aspects. Thus, gaining deeper insights into the parasite mitosis, its underlying dynamics and the involved proteins may reveal an entire array of novel targets. In this respect, the membrane occupation and recognition nexus protein 1 (MORN1) which is conserved among Apicomplexa may be a promising candidate. In Toxoplasma gondii MORN1 is associated with the spindle poles and the Inner Membrane Complex (IMC). Overexpression of TgMORN1 results in serious defects in nuclear segregation. In P. falciparum, MORN1 has been found to be solely expressed in schizonts suggesting a function during mitosis. To further elucidate Morn1 function in P. falciparum, affinity purified anti-PfMORN1-antibodies were used for stage specific Western blot and indirect immunofluorescence analysis. The anti-PfMorn1-antibody detected a single band of the expected size of 41.4 kDa. Highest PfMorn1 amounts were found in schizonts and segmenters consistent with its function in mitosis and its association with the IMC. However, contrary to previous studies PfMorn1 expression was also found in rings, which could be confirmed using a second antibody. These results are in good agreement with the microscopic examination. Segmenter stages showed a pattern that could be characteristic for an association with the IMC of merozoites. Rings, however, showed DNA associated MORN1-structures, located terminally at the crescent shaped DNA. The latter findings suggests that MORN1 may not only be crucial for the nuclear division, but may also be required in the earlier development of P. falciparum, making it even more suited as potential drug target.
Faisal Moin
Oman Medical College, Oman
Title: Co-existence of Dengue Fever & Malaria in thrombocytopenic patients presented with Acute febrile illness
Time : INF-02
Biography:
Abstract:
Background & Aim: Dengue fever and malaria both can present with thrombocytopenia and is regarded as a strong predictor of dengue fever. Thrombocytopenia is also considered criterion of disease severity, bad prognostic factor and its presence is associated with increase probability of malaria. The study aims to determine frequency of co-existence of dengue fever and malaria in thrombocytopenic patients presented with acute febrile illness in tertiary care hospital.
Method: Cross-sectional, observational study was conducted at the department of Emergency Medicine, Ziauddin University Hospital, Karachi from April 2013 to January 2014. A total of 159 patients meeting inclusion criteria were included in this study. 5 ml of blood by venupuncture in EDTA anti-coagulant for platelet count and preparing thick and thin films and 2 ml of blood in plain bottle for detection of dengue specific IgM was collected from all patients. Thick films are used to identify malarial parasites and thin films to identify specie. Dengue fever was diagnosed on positive dengue IgM. Co-existence was labeled as positive if malarial parasites and dengue IgM found to be present at the same time. This diffusion susceptibility test was used to determine susceptibility of bacterial agents to antibiotics. Data was analyzed by descriptive statistics using SPSS software version 19.
Results: Overall mean (±SD) age was 38.3±7.9 years, with Male to female ratio was 1.1: 1. Coinfections (Dengue and Malaria) were diagnosed in 5 (5.6%) of cases. From 5 cases, 3 (60%) were male and 2 (40%) were female. Mean (±SD) age of 5 positive cases of co-infection was 37.8±8.3 years.
Conclusion: Concurrent infections were found 5.6% in this study, however, this percentage is slightly low and special consideration should be given to the likelihood of co-infection with dengue and malaria.
Mohammed Hussein Eltoum Salih
Karary University, Sudan
Title: Impact of mass treatment and health education on the prevalence of Schistosomia haematobium among school children in four villages, White Nile State, Sudan
Time : INF-03
Biography:
Abstract:
This study was conducted to determine the prevalence of S. haematobium infection among school children from four villages namely Jadeed, Alandraba, Um Gaar and EL Shetabe in White Nile State, Sudan and to elucidate the impact of praziquantel on the reduction of the disease prevalence as well as the impact of health education on attitude, knowledge and behavior of children towards different aspects of the disease. The simple random technique was employed to choose school children of study villages. The effects of Praziquantel and health education were assessed prior and one year after launching parasitological, treatment and health education campaigns. The results revealed that the overall prevalence of S. haematobium infection in the four villages was 25%. The intervention using praziquantel showed a significant difference (P<0.05) and the prevalence of infection was reduced from 22% to Zero among Jadeed school children, from 47% to 9% among Um Gaar school children and from 29% to 5% among those of El Shetabe village, while increased from 1% to 5% among Alandraba school children.
Biography:
He, Abhishek Gupta, is a PhD research scholar at Department of Zoology, DN (PG) College, Meerut (UP), India. His research area is molecular taxonomy. He has expertise in molecular biology.
Abstract:
During the survey of freshwater fish Wallago attu, severe infection of known myxosporean species, Thelohanellus batae Lalitha kumari,1969 belonging to genus Thelohellnus Kudo, 1933 parasitizing gill filaments was found from Meerut District (UP), India. This species was described on the basis of morphology of plasmodia, histology findings on locations of plasmodia and DNA sequence data. Plasmodia of T. wallogoi are characterized in having spores measuring 11.8 to 6.7 µm, pyriform in valvular view and lenticular in sutural view having anterior end pointed and posterior end rounded with shell valves. Mature plasmodia are histozoic with single polar capsule measuring 7.2 to 3.0 µm, having polar filaments of 18 µm with 4 to 5 coilings. Histology reveal no pathological changes but severe infections. SEM revealed flat surface. Supplemented 18S rRNA gene sequence of T. batae did not show a close relationship with any other Thelohanellus spp., represented in gene bank.